2Başkent Üniversitesi, Transplantasyon ve Gen Bilimleri Enstitüsü, Ankara
3Gülhane Askeri Tıp Akademisi, Onkoloji Anabilim Dalı, Ankara OBJECTIVES
Acquired or intrinsic multiple drug resistance (MDR) is one of the limitations for successful chemotherapy. In this study, MDR mechanisms developed after paclitaxel and vincristine application in MCF-7 cell line were investigated.
METHODS
Paclitaxel and vincristine were applied to MCF-7 cell line in
stepwise manner. The resistance levels of the cells were determined
by XTT test. The levels of all of the genes in resistant
and sensitive cells were determined by microarray assay.
RESULTS
MCF-7/400nMPac and MCF-7/120nMVinc cells are 150- and
30-fold resistant compared to sensitive MCF-7. Upregulation
of MDR1 gene is found to be important in resistance. The
expression levels of the genes encoding detoxifying enzymes
were upregulated and some apoptotic gene levels were downregulated.
Changes in expression levels of metastatic genes,
some oncogenes, cell cycle, and tubulin-related genes were
found.
CONCLUSION
According to these results, change in expression level of
MDR1 gene seems to be important in resistance in MCF-7
cells.