2Department of Health Care Services, Vocational School of Health Services Atatürk University, Erzurum-Türkiye
3Department of Histology, Kafkas University Faculty of Veterinary Medicine, Kars-Türkiye DOI : 10.5505/tjo.2023.3974 OBJECTIVE
This experimental study was aimed to assess the positive or negative effects of the radioprotective agent amifostine (AMF) (WR-2721) on mucin 1 (MUC1) through immunohistochemical methods in preventing the damage that may occur in the prostate tissue after radiotherapy (RT).
METHODS
In the experimental period, 32 Sprague-Dawley male rats were divided into 4 groups: Untreated control
group, AMF group; 200 mg AMF was intraperitoneally administered to the rats in the second group for
3 days. The rats in the third group (RT-saline) were experimentally administered a total of 6 Gy including
2 Gy of RT in 3 fractions. The rats in the fourth group (RT - AMF) received totally 6 Gy RT and
200 mg/kg AMF for 3 days. The rats were sacrificed for histological assessments 4 weeks after RT. Triple
staining was performed to the tissues for histopathological examinations and immunohistochemical
localization of MUC1 in prostate tissue was determined using streptavidin-biotin peroxidase method.
RESULTS
Histopathologically, it was observed that the parenchymal and stromal cells of the prostate tissues in all
groups had a structurally normal appearance. Immunohistochemically, the MUC1 immunoreactivity
was weak in the parenchymal and stromal cells of the control group, and strong MUC1 secretion was
observed in the AMF group, AMF RT group, and RT group.
CONCLUSION
When considering the effects of AMF application on MUC1 secretion in the prostate tissue of rats
treated with RT, it is thought that the protective effect of AMF may vary in tissues, depending on the
dose applied and the number of fractions.