Correlation between Decreased Nuclear Factor Kappa Beta (NFKΒ), Kras, and Braf Genes" Expression and Enhanced Chemosensitivity of Human Squamous Cell Carcinoma Cells to 5-fluorouracil and/or Mesenchymal Stem Cells-derived Microvesicles
Iman Mahmoud ABOUSHADY1,Ghada Abdel Aziz ABDEL-LATİF2,Dina SABRY4,Sara El MOSHY1
1Department of Oral Biology, Cairo University Faculty of Dentistry, Cairo-Egypt
2Department of Oral Pathology, Suez Canal University Faculty of Dentistry, Ismailia-Egypt
3Department of Oral Basic and Clinical Sciences, Taibah University College of Dentistry, Madinah-Saudi Arabia
4Department of Medical Biochemistry and Molecular Biology, Cairo University Faculty of Medicine, Cairo-Egypt
DOI : 10.5505/tjo.2021.2714 OBJECTIVE
Oral squamous cell carcinoma (OSCC) comprises about 10% of head and neck cancer. 5-fluorouracil (5- FU) is commonly used for the treatment of OSCC. However, it has many limitations due to its identified side effects. Therefore, this work compared squamous cell carcinoma (SCC) cells" chemosensitivity to 5-FU, mesenchymal stem cells-derived microvesicles (MVs), and their combination.

Human SCC cell line (SCC152) was subjected to 5-FU or MVs or their combination for 24 h and 48 h. Inverted microscopic evaluation of apoptosis, MTT cell proliferation assay, DNA comet assay, and detection of NFKΒ, Kras, and Braf genes" expression were performed.

The combination group, compared to 5-FU or MVs treated groups, showed the most apparent apoptotic features. Cell proliferation was significantly decreased, while the tailed DNA% was significantly increased in the combination group versus either the 5-FU or MVs groups. The combination group showed a significant decrease in NFKΒ, Kras, and Braf genes" expression than the 5-FU or MVs treated groups. The correlation between cell proliferation and the studied genes showed a very strong positive linear relationship, while a strong negative linear relationship existed between cell proliferation and tailed DNA%.

The combination of MVs and 5-FU enhanced the genotoxicity and cytotoxicity of the SCC152 cell line compared to using either of them alone. Moreover, downregulation of NFKΒ, Kras, and Braf genes" expression was associated with enhanced apoptotic features, decreased cell proliferation, and enhanced tailed DNA%. Keywords : 5-Fluorouracil; braf; kras; microvesicles; NFK?; SCC152 cell line