2Department of Oral and Maxillofacial Surgery, Tehran University of Medical Sciences Faculty of Dentistry, Tehran-Iran
3Department of Head and Neck Surgical Oncology and Reconstructive Surgery, The Cancer Institute, Tehran University of Medical Sciences Faculty of Medicine,Tehran-Iran DOI : 10.5505/tjo.2021.2702 OBJECTIVE
MicroRNAs (miRs) are small, non-coding RNAs that are involved in tumor development and progression in multiple cancers with the action as oncomiRs or tumor suppressor miRs. Therefore, miRNAs have become potential biomarkers in the diagnosis and treatment of cancer. The previous studies have revealed that miR7111-5p and miR6870-5p are abnormally expressed in various types of cancer but in oral squamous cell carcinoma (OSCC) has not been elucidated. The main objective of our study was to evaluate the expression alternations of miR7111-5p and miR6870-5p as well as serum response factor (SRF) in OSCC tissues compared with adjacent normal oral epithelial tissues.
METHODS
SRF was identified as a potential target gene of miR7111-5p and miR6870-5p using the TargetScan, Mirwalk,
and Mirdb miRNA-target prediction tools. SRF, miR7111-5p, and miR6870-5p expression levels
were examined by RT-qPCR in OSCC compared with normal tissues.
RESULTS
Our results showed that miR7111-5p and miR6870-5p were significantly downregulated in 30 pairs of
OSCC tissue samples (p<0.05) relative to adjacent normal oral tissues. In the present study, we found that
the high expression level of SRF was negatively correlated with the down regulation of miR7111-5p and
miR6870-5p and was statistically correlated with the tumor stage.
CONCLUSION
miR7111-5p, miR6870-5p, and SRF may be potential prognostic biomarkers for OSCC diagnosis and
monitoring.